Shim-pack VP Series - Features
HPLC Column
Strict Manufacturing Uniformity
Balance of Number of Theoretical Plates and Column Pressure
Shim-pack VP series achieves a higher number of theoretical plates while maintaining low pressure. It provides superior performance shown by NTP per 1MPa.
Excellent Peak Shape
Smooth Transfer of Methods from High-Speed Analysis to Conventional Analysis
By using the Shim-pack VP series of conventional columns in combination with the Shim-pack XR series of high-speed columns, which offer the equivalent separation performance, it is possible to perform method development via high-speed analysis, and then smoothly transfer to conventional analysis. Utilizing these columns with Nexera Method Scouting provides strong support for method development.
Conditions | |||
Column | : | Top: |
Shim-pack VP-ODS
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-34937-91) |
Bottom: |
Shim-pack XR-ODS
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-41606-92) |
||
Mobile Phase | : | A) 0.1 % formic acid B) Acetonitrile A/B = 8/2 (v/v) |
|
Flow Rate | : | Top: | 1.0 mL/min |
: | Bottom: | 1.0 mL/min |
Relative Retention Times
Peak | Shim-pack VP-C8 | Shim-pack XR-C8 |
1. Sulfadiamine | 1.000 | 1.000 |
2. Sulfamerazine | 1.252 | 1.238 |
3. Sulfadimidine | 1.537 | 1.509 |
4. Sulfamethoxazole | 2.809 | 2.699 |
5. Sulfaquinoxaline | 5.668 | 5.432 |
Analysis of Fat-Soluble Vitamin
Conditions | |||
Column | : | Top: |
Shim-pack VP-C8
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59927-91) |
Bottom: |
Shim-pack XR-C8
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59902-92) |
||
Mobile Phase | : | Methanol | |
Flow Rate | : | Top: | 1.0 mL/min |
: | Bottom: | 1.0 mL/min |
Relative Retention Times
Peak | Shim-pack VP-C8 | Shim-pack XR-C8 |
1. Retinol | 1.000 | 1.000 |
2. α±−tocopherol | 1.509 | 1.531 |
Analysis of Fat-Soluble Vitamin
Conditions | |||
Column | : | Top: |
Shim-pack VP-C8
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59927-91) |
Bottom: |
Shim-pack XR-C8
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59902-92) |
||
Mobile Phase | : | Methanol | |
Flow Rate | : | Top: | 1.0 mL/min |
: | Bottom: | 1.0 mL/min |
Relative Retention Times
Peak | Shim-pack VP-C8 | Shim-pack XR-C8 |
1. Retinol | 1.000 | 1.000 |
2. α±−tocopherol | 1.509 | 1.531 |
Batch Analysis of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs)
Conditions | |||
Column | : | Top: |
Shim-pack VP-Phenyl
(150 mmL. × 4.6 mmI.D., 5 μm)
(P/N: 228-59928-91) |
Bottom: |
Shim-pack XR-Phenyl
(50 mmL. × 3.0 mmI.D., 2.2 μm)
(P/N: 228-59904-92) |
||
Mobile Phase | : | A) 20 mmol/L Phosphoric acid (Na) buffer (pH 2.5) B) Acetonitrile A/B = 3/7 (v/v) |
|
Flow Rate | : | Top: | 1.0 mL/min |
: | Bottom: | 1.0 mL/min |
Relative Retention Times
Peak | Shim-pack VP-Phenyl | Shim-pack XR-Phenyl |
1. Piroxicam | 1.000 | 1.000 |
2. Ketoprofen | 1.480 | 1.483 |
3. Diflunisal | 2.233 | 2.172 |
4. Diclofenac | 2.949 | 2.916 |
5. Indomethacin | 3.263 | 3.262 |
6. Ibuprofen | 3.470 | 3.501 |
Specifications
Shim-pack VP-ODS | Shim-pack VP-C8 | Shim-pack VP-Phenyl | |
Particle Size (µm) | 5 | 5 | 5 |
Pore Size (nm) | 12 | 12 | 12 |
Surface Area (m2/g) | 410 | 410 | 410 |
Carbon Loading | 20% | 12.5% | 12.3% |
Pressure Tolerance (MPa) | Approx. 20 | Approx. 20 | Approx. 20 |
Pore Volume (mL/g) | 1.25 | 1.25 | 1.25 |
End-capping | Yes | Yes | Yes |
Bonding Type | Monomeric | Monomeric | Monomeric |
pH range | 2-7.5 | 2-7.5 | 2-7.5 |
USP Code | L1 | L7 | L11 |
* Appearance and specifications are subject to change without notice.