Shim-pack Scepter LC Columns - Features : Shimadzu (Asia Pacific)

Eight stationary phases and 3 column hardware types for extensive sample coverage
Utilize excellent stability & performance under a wide range of LC conditions
Superior Sensitivity and Separation Performance in metal-coordinating compounds
Wide-pore reversed-phase column optimized for analysis of middle-molecular-weight compounds and proteins

Eight stationary phases and 3 column hardware types for extensive sample coverage

Shim-pack Scepter Chemistries

Shim-pack Scepter	Reveresd Phase							HILIC
Shim-pack Scepter	C18-120	C18-300	HD-C18-80	C8-120	C4-300	Phenyl	PFPP	Diol-HILIC
Ligand Type	Trifunctional C18	Trifunctional C18	Trifunctional C18	Trifunctional C8	Trifunctional C4	Trifunctional Phenylbutyl	Trifunctional Pentafluorophenylpropyl	Trifunctional Dihydroxypropyl
Ligand Type	General Purpose	General Purpose for Large Molecules	High Density Type for Increased Retention	Trifunctional C8	Trifunctional C4	Trifunctional Phenylbutyl	Trifunctional Pentafluorophenylpropyl	Trifunctional Dihydroxypropyl
Particle	Organic Silica Hybrid
Particle Size	1.9 μm, 3 μm, 5 μm
Pore Size	12 nm (120Å)	30 nm (300Å)	8 nm (80Å)	12 nm (120Å)	30 nm (200Å)	12 nm (120Å)
End Capping	Proprietary						None
Carbon%	20	N.D.	25	17	N.D.	17	15	12
pH Range	1 - 12				1 - 10		1 - 8	2 - 10
100 % Aqueous Condition	YES	YES	×	×	YES	YES	YES	—
USP Classification	L1	L1	L1	L7	L26	L11	L43	L20

Shim-pack Scepter Column Hardware

	Scepter	Scepter Claris	Scepter [metal-free]

Wetted materials for body	Stainless steel	Bioinert coating	PEEK
Wetted materials for frit	Stainless steel	Bioinert coating	PEEK

Utilize excellent stability & performance under a wide range of LC conditions

With excellent stability under a wide range of LC conditions, Shim-pack Scepter LC columns are effective for method scouting combining mobile phase pH and organic modifier.

【 Comparison of Chromatograms using Gradient condition with Acetonitrile 】

	C18	HD-C18	C8	Phenyl	PFPP
pH 2.9
pH 6.1
pH 9.9					Outside of recommended pH

Analytes

1. Saccharin (pKa = 2.2)

2. Dextromethorphan (pKa = 8.3)

3. Amitriptyline (pKa = 9.4)

4. N-Butyl paraben (pKa = 8.3)

5. Ibupurofen (pKa = 4.4)

Superior Sensitivity and Separation Performance in metal-coordinating compounds

Shim-pack Scepter Claris

Shim-pack Scepter Claris features a column body with a newly-developed bioinert coating packed with Scepter series stationary phases.

Bioinert coating is applied to the column body and stainless steel frit.
Ideal for analysis of metal-coordinating and hydrophobically adsorbing compounds such as nucleic acids, proteins, and lipids
Outstanding pH and lifetime stability due to Scepter organic silica hybrid packing

Superior Sensitivity and Separation Performance in Nucleic Acid Analysis

Shim-pack Scepter Claris C18-120 with the bioinert coating and Scepter C18-120 with traditional stainless steel hardware were compared in this example of analysis of synthetic oligonucleotide. Results from Claris were highly sensitive and reproducible from the first injection, with no loss of sample signal. Scepter C18-120 in a stainless steel column body produced low sensitivity results and showed adsorption from the first sample injection.

Wide-pore reversed-phase column optimized for analysis of middle-molecular-weight compounds and proteins

Shim-pack Scepter C18-300, C4-300

Shim-pack Scepter C18-300 and C4-300 are wide-pore organic silica hybrid reversed phase columns recommended for the separation of proteins such as monoclonal antibodies and mid-sized molecules such as oligonucleic acids and peptides that may not be retained on 100-120Å pore size columns due to size-exclusion effects. The organic silica hybrid base material is highly stable even at high temperatures under acidic and basic mobile phase conditions. Pore sizes are optimized for large molecular weight compounds and dispersed uniformly in the column, resulting in symmetrical peak shapes and increased resolution of antibodies and nucleic acids compared to a smaller pore size column. Shim-pack Scepter C18-300 and C4-300 are also effective for high-sensitivity LC/MS analysis, producing good peak shapes even under weak ionpairing conditions using a formic acid mobile phase.

	C18-300	C4-300
Ligand Type	Trifunctional C18	Trifunctional C４
Ligand Type	Generic Purpose Type	Generic Purpose Type
Particle Size	1.9, 3, 5 μm
Pore Size	300Å
End Capping	Proprietary
pH Range	1-12
100% aqueous condition	YES
USP Classification	L1	L26

Peptide Analysis Example

Shim-pack Scepter C18-300 and C4-300 made with 300 Å (30 nm) pore size packing material. The larger pore size is recommended for the analysis of mid-sized and large-sized molecules with molecular weights above 5000 to enable retention and avoid size-exclusion effects. This analysis of a mixed sample of four different peptides and proteins demonstrated good peak shapes and favorable separation of insulin and ribonuclease A due to the pore size that was large enough to allow proper diffusion of these large molecular weight compounds.