Simple PCR from Microorganisms

Simple PCR from Microorganisms

Polymerase chain reaction (PCR) was performed on microorganisms using Ampdirect® (buffer) to suppress the actions of PCR inhibitors, such as proteins and polysaccharides, and NovaTaqTM (recommended enzyme).
→ Ampdirect® allows quick and convenient PCR, without DNA purification.

Gene Amplification Reagent Ampdirect^TM

• DNA present in blood can be amplified directly.
• No DNA extraction is required.
• Thus, risk of cross contamination and/or procedural error is dramatically reduced.
• Only small volumes of blood are necessary (0.5-1μl ).
• Various anticoagulants (including sodium citrate, dipotassium EDTA, and sodium heparinate) can be used.
• Blood (citrated or EDTA-treated blood is recommended) stored frozen for long periods can be analyzed.
• Simplifies PCR methods resulting in its use in a broad range of routine applications including mass screening.