Genetic Inspection of Food | Rapid Meat Species Identification
Identification of Meat Type by DNA/RNA Analysis System (Meat Species Identification)
Recent concerns over food safety have raised consumer awareness and led to demands for accurate, easy, and rapid discrimination between the types of meat contained in food products.
Microchip Electrophoresis System for DNA/RNA Analysis can rapidly identify the meat species. Chicken, beef, mutton, pork, and horse meat were analyzed.
Results
The following fragments were clearly separated to distinguish between the respective meat species: 218 bp derived from chicken, 268 bp derived from beef, 331 bp derived from mutton, 359 bp derived from pork, and 430 bp derived from horse.
Analysis Results for Five Meat Species
Analytical Procedure
Analytical Instrument: MCE-202 "MultiNA"
Analysis Mode: DNA-500 Premix
PCR Primer:
Based on the paper of Matsunaga, et. al. (Journal of Japanese Food Science and Engineering, 46(3), 187, 1999), some modifications were made to the base array.
- AmpdirectR(by Shimadzu Corporation)P/N 241-08890-92
- AmpdirectR (by Shimadzu Corporation) P/N 241-08890-92
- DNA-500 Reagent Kit for MultiNA (by Shimadzu Corporation) P/N 292-27910-91
- DNA-500 Reagent Kit for MultiNA (by Shimadzu Corporation) P/N 292-27910-91
- SYPRR Gold nucleic acid gel stain (by Invitrogen Corporation) S-11494
- 25bp DNA ladder (by Invitrogen Corporation) 10597-011
Microchip Electrophoresis System for DNA/RNA
DNA and RNA samples are separated by size by the electrophoresis system using a microchip so that the size of nucleic acid (DNA/RNA) samples is verified and approximately quantitated. The microchip achieves an electrophoresis system capable of speedily conducting electrophoresis separation, and a fluorescent detector ensures that analysis is performed to high sensitivity and, moreover, fully automatically.