Live Online LCMS Training
LCMS Courses
Principles, Operation and Maintenance of LCMS-2050 with Labsolutions
The course is dedicated for LCMS-2050 users covering from principles and instrumentation of ultrafast LCMS (UFMS) to hands-on sessions for well operation of the system and data analysis using LabSolutions. Interface maintenance and MS tuning are included.
Duration:
2 Day(s)
Prerequisites:
Fundamental knowledge of HPLC and analytical chemistry are required.
Topics Included:
- Principles and instrumentation of single quadrupole LC/MS and DUIS interfacing techniques
- LabSolutions for LCMS Software & Operation
- Qualitative and quantitative analysis using Scan and SIM modes
- New feature: Mass-it, i-peakFinder
- DUIS interface maintenance and MS auto-tuning
Principles, Operation and Maintenance of Triple Quadrupole with LabSolutions for LCMS-8045/8050/8060NX
The course is designed for users of Shimadzu triple quadrupole LC/MS/MS series LCMS-8045, LCMS-8050 and LCMS-8060NX. It covers from ultrafast TQ principles and instrumentation to hand-on sessions for well-operation of the systems using LabSolutions. Quantitative analysis method based on MRM mode and optimization of interface and MS parameters are focused. Interface maintenance and TQ tuning are included.
Duration:
2 Day(s)
Prerequisites:
Fundamentals knowledge of HPLC and analytical chemistry are required.
Topics Included:
- Principles and instrumentation of UFMS (TQ)
- MRM optimization using Auto MRM optimization program
- MRM method set up and quantitation of drug molecules
- LabSolutions Post-run for data analysis
- Interface parameters setting and optimization
- Interface maintenance and MS tuning
LabSolutions Insight for Multi-data Quantitative Analysis
This module focuses on multi-analytes MRM data analysis using advanced s/w LabSolutions Insight for LCMS.
Duration:
1 Day(s)
Prerequisites:
Attended LCMS-A102N
Topics Included:
- LabSolutions Insight—advantages and features
- Insight workflow:
- Data view
- Method edit and update
- Flag and flagging criteria
- Review of results, approve or reject
- Reporting
Principles, Operation and Maintenance of LCMS-9030/9050 – HRAM & MS/MS Applications
This course is designed for users of LCMS-9030/9050, Quadrupole Time-of-Flight Mass Spectrometry (Q-TOF). It includes lectures and practical sessions on the principles of HRMS, instrumentation of hybrid Q-TOF system, operation of data acquisition and data analysis for qualitative and quantitative analysis.
Duration:
4 Day(s)
Prerequisites:
For LCMS-9030 users only.
Topics Included:
- Theory and principle of HRAM analysis on LC-Q-TOF hybrid MS/MS
- Instrumentation of LCMS-9030/9050
- Data acquisition mode, MS, MS/MS, DDA and DIA
- Mass calibration for accurate mass analysis - unique techniques
- LabSolutions workstation and LabSolutions Insight Explore (Brief)
- Method setup for various applications
- Maintenance of interface, CDS and full tuning
LabSolutions Insight Explore for Untargeted Screening DIA/DDA
This advanced module is for LCMS-9030/9050 users for HRAM data analysis for various purposes. The LabSolutions Insight—Explore s/w suite enables processing effectively MS, MS/MS, DDA and DIA data for identification, formula prediction, structural elucidation, precursor deconvolution, database search etc.
Duration:
1 Day(s)
Prerequisites:
Attended LCMS-A105
Topics Included:
- LabSolutions Insight Explore suite — advantages and features
- Insight workflows:
- Targeted screening by HRAM and library search by DDA
- Untargeted screening and unknown ID by DIA
- Structural elucidation by Assign and database search
- Confirmation of compounds by formula prediction and library search
Method Development of LC/MS/MS for Quantitative and Qualitative Analyses
This advanced module is designed for LC-MS/MS (TQ) users who wish to further develop skills in method development. The course focuses on method development strategy and approach for high sensitivity and multi-target quantitative analysis using MRM mode as well as qualitative analysis using modes like product ion scan, precursor ion scan, neutral loss scan.
Duration:
2 Day(s)
Prerequisites:
Attended LCMS-A102N.
Topics Included:
- Principles and strategies of triple quadrupole LC/MS/MS
- Development of quantitative MRM method with focusing on sensitivity, speed, and overall performance
- Qualitative analysis methods and applications of triple quadrupole LC/MS/MS for various application tasks
Quantitative Bioanalysis of Drugs by LC/MS/MS – Method Development and Validation Procedure
An advanced course designed for LC/MS/MS users to develop MRM-based method for quantitative analysis of drugs in biological samples. The course introduces the requirements of bio-analysis methods for BA/BE study, clinical analysis, and drug discovery, focusing on the design and development of appropriate methods for different drugs and different requirements. Sample pretreatment methods are also described and reviewed.
Duration:
2 Day(s)
Prerequisites:
Attended LCMS-A102N.
Topics Included:
- Scopes of bio-analysis by LC/MS/MS methods
- Design and development of MRM-based quantitative method for biological samples
- Pretreatment of biological samples for LC/MS/MS analysis
- Procedure of method development and validation
QuEChERS Sample Pretreatment and LC/MS/MS Method for Food safety Analyses
An advanced course designed for LC-MS/MS users to develop MRM-based method for quantitative analysis of veterinary drugs in food samples. The course focuses on QuEChERS sample pretreatment procedure with hands-on lab session for food samples, followed by MRM method analysis.
Duration:
2 Day(s)
Prerequisites:
Attended LCMS-A102N.
Topics Included:
- Procedure of QuEChERS sample pretreatment
- Hands on session for pretreatment of food samples
- MRM-based quantitative method for veterinary drug samples for LC/MS/MS analysis
- Evaluation of matrix effect and recovery
Gelatin Analysis for HALAL Testing by LC-MS/MS Method
Gelatin is used in foods, cosmetics and pharmaceutical products for its gel forming properties. This advanced module shows an end-to-end procedure for detection and differentiate between bovine gelatin and porcine gelatin in processed food and pharmaceutical capsule by LC-MS/MS method.
Duration:
2 Day(s)
Prerequisites:
Attended LCMS-A102N
Topics Included:
- Principle of targeted proteomics for detection of specific peptides (markers) of gelatins from different animal species
- MRM method development procedure with Skyline s/w
- Sample preparation procedure and practice
- LC-MS/MS method of digested samples and result